Confirming the arrest, the state police commissioner, Ibrahim Idris, said the three journalists were detained for saying on a radio show that polio immunisations were anti-Islam western conspiracy to cause infertility in women.
Mohammed Gama, Yakubu Fagge and an intern, attached to Wazobia FM in the city, allegedly made the statement two days before the 9 workers administering the vaccines were killed.
The gunmen on tricycles shot dead the vaccinators in two separate attacks.
Although no one claimed responsibility, Islamist militant group Boko Haram, is known to have condemned the use of western education.
It is feared that the killings could hamper efforts by international health organisations to rid the north of the polio virus.